非洲爪蟾（Xenopus laevis）是发育生物学、细胞与分子生物学的经典模式动物。约翰·格登（John Gurdon）凭借爪蟾核移植实验揭示了细胞核的全能性，因此荣获 2012 年诺贝尔生理学或医学奖。随着研究深入，对爪蟾相关资源的需求持续增长，建立集约化的爪蟾资源共享平台成为国内科研群体的迫切需要。然而，国内长期缺少一个稳定、专业的爪蟾资源平台，这促使我们创立了爪蟾资源中心。The African clawed frog (Xenopus laevis) is a classic model in developmental, cell and molecular biology. John Gurdon's nuclear-transfer experiments revealed nuclear totipotency and earned the 2012 Nobel Prize in Physiology or Medicine. As research deepens, the growing demand for centralized Xenopus resources led us to establish this center.
建设背景Background
非洲爪蟾作为脊椎动物模式生物具有独特优势：体外受精与发育、产卵量大（每次可达 200–1000 枚）、胚胎相对透明、遗传工具完备。然而与斑马鱼、小鼠相比，国内爪蟾资源分散，养殖技术、实验规范和数据资源尚缺乏整合，制约了其在更多研究机构的推广应用。As a vertebrate model, Xenopus offers external fertilization, large egg clutches (200–1,000 per spawn), transparent embryos and a rich genetic toolkit. However, compared with zebrafish and mice, domestic Xenopus resources are fragmented — husbandry know-how, experimental protocols and data remain scattered, limiting broader adoption.
网站主要功能What this site provides
- 资源信息平台：介绍爪蟾模式动物特点、基因组数据、典型实验方法与技术规程；Resource hub: model biology, genome data, experimental methods and SOPs;
- 实验动物供应：建设标准化养殖基地，为研究人员提供稳定的爪蟾成体、蝌蚪及卵母细胞；Animal supply: standardized husbandry facility providing adults, tadpoles and oocytes;
- 一站式技术服务：卵母细胞异源表达、TEVC 电生理、单细胞提取、发育毒理等委托实验；One-stop services: oocyte heterologous expression, TEVC, single-cell isolation, developmental toxicity;
- 学术交流平台：整合国内外研究资讯，支持中国爪蟾研究者之间以及与国际同行的学术交流。Academic exchange: aggregating domestic and international news to connect the Chinese Xenopus community.
建站意义Why it matters
- 降低爪蟾实验的入门门槛，推动爪蟾资源在更多科研机构的应用；Lower the barrier to entry, enabling more labs to adopt Xenopus;
- 提升实验规范化水平，促进不同实验室间数据的可重复性；Improve experimental standardization and reproducibility across labs;
- 加强国内外爪蟾研究群体的协作与信息共享。Strengthen collaboration and information sharing within the global Xenopus community.
正如我们的口号：我们一起打造一个专业的爪蟾资源平台！As our slogan goes: Let's build a professional Xenopus resource platform together!
参考文献References
- Gurdon JB (2012) The egg and the nucleus: a battle for supremacy. Nobel Lecture. Angew Chem Int Ed 52(12):3177–3180.
- Harland R & Grainger R (2011) Xenopus research: metamorphosed by genetics and genomics. Trends Genet 27(12):507–515.
- Gurdon JB & Hopwood N (2000) The introduction of Xenopus laevis into developmental biology. Int J Dev Biol 44(1):43–50.