常用缓冲液配方

2019-03-05 11:33:47

100X Steinberg’s Stock

119g HEPES

34g NaCl

2.06g magnesium sulfate•(7 H2O)

0.8g calcium nitrate•(4 H2O)

0.5g potassium chloride

bring to 1L using milliq H2O and Adjust to pH 7.5

Steinberg’s (working solution)

10 L milliq water

100 ml 100x steinberg’s stock

500 ul pen/strep pH 7.4

MS222

500 ml steinberg’s solution

0.1g MS222 powder pH 7.4

MBS-H (Modified Barth solution) 10x/1l

88mM NaCl 51.4g

1mM KCl 0.75g

2.4mM NaHCO₃2.0g

0.82mM MgSO₄x7H₂O 2.0g

0.33mM Ca(NO₃)₂x4H₂O 0.78g

0.41mM CaCl₂ x2H₂O 0.6g

10mM HEPES 23.8g

10mg/ml Streptomycin Sulfate and Penicillin 1ml 10mg/ml stock (-20, antibiotics)

adjust pH to 7.4 with NaOH

sterile filter buffer

NOTE: The working dilution of MBS-H is 0.1x. This means that the 10x stock should be diluted 100x to get the final working concentration.

10x PBS pH 7.2

1 L 3 L

1,37M NaCl 80.06g 240.18g

30mM KCl 2.25g 6.75g

65mM Na₂HPO₄ x H₂O 9.23g 27.68g

15mM KH₂PO₄2.05g6.15g

adjust pH with NaOH to pH 7.2